BD Prepared Media Tubes BBL™ Tube, Size K, Urea Agar Complete Christensen, 10 slants/pk 221096

Features & Benefits:

• Urea Agar is used for the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production.
• Urea Agar was devised by Christensen for use as a solid medium for the differentiation of enteric bacilli.
• It differentiates between rapid urease-positive Proteeae organisms (Proteus spp., Morganella morganii subsp. morganii, Providencia rettgeri, and some Providenciastuartii) and other urease-positive organisms: Citrobacter, Enterobacter and Klebsiella and bacteria other than Enterobacteriaceae; i.e., some Bordetella and Brucella species.
• The base is also supplied as a filter-sterilized 10X concentrated solution in tubes for use in preparing Urea Agar slants in the user's laboratory.
• The urea medium of Rustigian and Stuart3 is particularly suited for the differentiation of Proteus species from other gram-negative enteric bacilli capable of utilizing urea;1 the latter are unable to do so in Urease Test Broth because of limited nutrients and the high buffering capacity of the medium.
• To provide a medium with greater utility, Urea Agar was devised by Christensen1 with peptone and dextrose included and reduced buffer content to promote more rapid growth of many of the Enterobacteriaceae and permit a reduction in incubation time.
• When organisms utilize urea, ammonia is formed during incubation which makes the reaction of these media alkaline, producing a pink-red color.
• Consequently, urease production may be detected by the change in the phenol red indicator.
• Additional Quality Control:
  • Examine tubes as described under "Product Deterioration."
  • Visually examine representative tubes to assure that any existing physical defects will not interfere with use.
  • Determine the pH potentiometrically at room temperature for adherence to the specification of 6.8 ± 0.2.
  • Incubate uninoculated representative tubes at 20-25 °C and 30-35 °C and examine after 7 days for microbial contamination.
• Tubes with tight caps should be opened carefully to avoid injury due to breakage of glass.
• Certifications/Compliance - CE, BAM, CCAM, ISO, USDA.
• Format - Liquid.
• Media Format - Pancreatic digest of Gelatin 1g/L, Dextrose 1g/L, Sodium Chloride 5g/L, Potassium Phosphate 2g/L, Urea 20g/L, Phenol red 0.012g/L, Agar 15g/L.
• Packaging - Glass Tube with Cap.
• Storage Requirements - 2° to 8°C.
• Color - Yellow.
• Media Type - Urea Agar.

Application:

• For Use With differentiation of organisms, especially Enterobacteriaceae, on basis of Urease production.