BD BBL™ Prepared Media Trypticase™ Soy Agar with 5% Sheep Blood (Trypticase™ Soy Agar II™) Plate, 20/pk 221239

Features & Benefits:

• The nutritional composition of Trypticase Soy Agar has made it a popular medium, both unsupplemented and as a base for media containing blood.
• Trypticase Soy Agar with 5% or 10% Sheep Blood is extensively used for the recovery and cultivation of fastidious microbial species and for the determination of hemolytic reactions which are important differentiating characteristics for bacteria, especially Streptococcus species.
• Plates labeled "deep fill" contain an additional volume of medium to reduce the effects of drying during prolonged incubation.
• The combination of casein and soy peptones in the Trypticase Soy Agar base render the medium highly nutritious by supplying organic nitrogen, particularly amino acids and larger-chained peptides.
• Trypticase Soy Agar with 5% Sheep Blood (TSA II) provides excellent growth and beta hemolysis by Streptococcus pyogenes (Lancefield group A) and also provides excellent growth and appropriate hemolytic reactions with other fastidious organisms.
• The CAMP test, which only is performed on TSA II, is based on the formation of a zone of synergistic hemolysis at the junction of perpendicular streak inocula of Staphylococcus aureus and group B streptococci.
• The reaction is caused by the sphingomyelinase (beta-toxin) of S. aureus reacting with sphingomyelin in the sheep erythrocyte membrane to produce ceramide. A non-enzymatic protein (CAMP protein), produced by S. agalactiae, binds to the ceramide and leads to disorganization of the lipid bilayer of the sheep erythrocyte membrane resulting in complete lysis.
• Additional Quality Control:
  • Examine plates as described under "Product Deterioration."
  • Visually examine representative plates to assure that any existing physical defects will not interfere with use.
  • Determine the pH potentiometrically at room temperature for adherence to the specification of 7.3 ± 0.2 for catalog numbers 221239 and 221261 and 7.4 ± 0.2 for catalog number 221162.
  • Note the firmness of plates during the inoculation procedure.
  • Incubate uninoculated representative plates at 35 ± 2 °C for 72 h and examine for microbial contamination.
• Ready for immediate use.
• Certifications/Compliance - ISO/DIS, 11014.
• Packaging - Plate.
• Media Format - Pancreatic digest of Casein 14.5g/L, Papaic digest of Soybean Meal 5g/L, Sodium Chloride 5g/L, Agar 14g/L, Growth Factors 1.5g/L, Defibrinated Sheep Blood 5% or 10%
• Storage Requirements - 2-8°C.
• ShelfLife - 105 day

Application:

• For Use With Cultivation of fastidious microorganisms and visualization of hemolytic reactions.